Epi眼 eye irritation assays have been incorporated into toxicology laboratories across the world. 直接的协议和简单但高度预测的终点, 在整个美容过程中，常规使用眼表分析, 个人护理, 化工和制药行业. Developed more than 20 years ago, Epi眼 has been used for testing thousands of materials. Whether scientists need a risk assessment assay for the irritation potential of an ocular delivered material or the hazard assessment of chemical being transported across the world, Epi眼是测试系统的选择，以满足您的眼科测试需求.
眼部过敏测试
外眼刺激试验(经合组织TG 492)
The Epi眼 EIT is an in vitro hazard assessment assay that has the ability to differentiate materials that are ocular non-irritants from materials that are not ocular irritants. The EIT is applicable to a broad range of organic and inorganic chemicals, and is accepted as 经合组织TG 492.
Watch a video on how to perform the Epi眼 Eye Irritation Test (EIT – 经合组织TG 492):
Epi眼亚雨超温和测试
The Epi眼 Ultra-mildness Test is an in vitro risk assessment assay routinely utilized for mild materials for which the Draize test is insensitive. This highly reproducible assay allows for quantifiable discrimination among mild, 温和的, 以及最温和的产品配方.
Epi眼 MTT ET-50 (Neat)
This in vitro risk assessment assay is based on the effective time at which a material (applied neat) causes a 50% reduction in tissue viability (ET-50). 基于ET-50, the test article is categorized into one of 4 classifications ranging from non-irritating to severe/extreme which correspond to groupings of Rabbit Draize Eye Scores (MMAS).
Epi眼 MTT ET-50(稀释)
类似于MTT ET-50 Neat方法, this in vitro risk assessment assay is based on the effective time at which a material (diluted to 20% in water) causes a 50% reduction in tissue viability (ET-50). 基于ET-50, the test article is categorized into one of 4 classifications ranging from non-irritating to severe/extreme which correspond to groupings of Rabbit Draize Eye Scores (MMAS). This method is recommended for surfactant-based solutions and is applicable to water-soluble materials with a specific gravity of > 0.95.
浏览我们的 参考图书馆 看看我们的眼外组织是如何用于这些研究领域的.
组织:
工具: Standard Epi眼™ kit (OCL-200) consists of 24 tissues, each tissue 9 mm in diameter. (组织“套件”包含组织, 少量培养基:少量培养基, and plasticware; contact yobo体育 for specific kit contents.)
基质: 使用无涂层的9毫米ID单孔组织培养板刀片(e.g. Millicell PCF, Nunc聚碳酸酯单孔组织培养板插入物.
文化: 在气液界面.
组织学: 细胞层5-8层(基底层至顶端表面).
角质化: 没有一个.
很多数字: Tissue lots produced by each technician for each week are assigned a specific lot number. Typically, there are multiple lot numbers for any given week’s tissue production. A letter of the alphabet is appended to the end of the lot number to differentiate between individual kits within a given lot of tissues. 所有的组织套件在细胞方面都是相同的, medium, 处理, 培养条件, 等.
装运: 在4°C的培养基补充，琼脂糖凝胶在24孔板.
装运: 每星期一. 如有特殊要求，星期四装运也可以.
交付: 周二上午通过联邦快递优先服务(美国). 美国以外:周二至周四，取决于地点.
保质期: Including time in transit, tissues may be stored at 4°C for up to 4 days prior to use. 然而, extended storage periods are not recommended unless absolutely necessary. 除了, the best reproducibility will be obtained if tissues are used consistently on the same day, e.g. 星期二下午或之后在4°C存放过夜(星期三上午).
替代组织:
ocl - 200沛富: 不组织. 在最后3天的培养中，从培养基中删除庆大霉素. 客户接收200毫升OCL-200-ASY-AFAB培养基.
ocl - 200等于off: Anti-fungal-free组织. 在最后3天的培养中，从培养基中删除两性霉素. 客户接收200毫升OCL-200-ASY-AFAB培养基.
ocl - 200 afab: 抗生素,anti-fungal-free组织. For last 3 days of culture, gentamicin and Amphotericin are omitted from culture medium. 客户接收200毫升OCL-200-ASY-AFAB培养基.
ocl - 200: 单眼外组织植入.
ocl - 200 - frzn - ea: 经过3次冻融循环的单个OCL-200组织. 设计用于MTT自动还原测试的控制.
ocl - 212: 半试剂盒(12个培养液，标准培养液24个).
ocl - 606: 除了每包6张纸巾外，与OCL-200相同, 每个组织在一个更大的细胞培养皿中培养(直径22毫米).
细胞:
类型: 正常人表皮角质形成细胞(NHEK).
基因组成: 单一捐赠者.
来自: Neonatal-foreskin组织.
筛选: HIV，乙型肝炎，丙型肝炎，支原体.
介质:
基础培养基: 杜尔贝科改良鹰型介质(DMEM).
生长因子/激素: 表皮生长因子, 胰岛素, hydrocortisone and other proprietary stimulators of non-keratinizing epithelial differentiation.
血清: 没有一个.
抗生素: 庆大霉素5µg/ml(庆大霉素正常水平的10%).
抗真菌剂:两性霉素b0.25µg / ml.
酸碱指示剂: 酚红.
选择: 苯酚red-free, 不需抗生素, anti-fungal-free, 或者不含氢化可的松的培养基和组织. 代理商至少在装运前3天被移除.
维护中: 大多数使用Epi眼™的检测都是在24小时内进行的. 然而, 长时间的实验, the tissue can be maintained in OCL-200-MM (identical to Epi眼™ assay medium).
质量控制和无菌:
目视检查: 所有组织都要进行目视检查，如果发现了物理缺陷, 组织被拒绝装运.
最终用途测试: 组织暴露于0.3% Triton X-100 5 20 60分钟. The time of exposure required to reduce the tissue viability (ET-50) using the MTT assay is determined (See yobo体育 Epi眼TM ET-50 protocol) for each lot of tissue. ET-50的值必须在1996 Epi眼™数据库的范围内:12.2-37.5分钟. 客户实验室中的ET-50通常与yobo体育的结果非常相似.
不育: 在整个生产过程中使用的所有培养基都要进行无菌检查. Maintenance medium is incubated with and without antibiotics for 1 week and checked for sterility. The agarose gel from the 24-well plate used for shipping is also incubated for 1 week and checked for any sign of contamination.
筛查病原体: All cells are screened and are negative for HIV, hepatitis B and hepatitis C using PCR. 然而, no known test method can offer complete assurance that the cells are pathogen free. 因此, these products and all human derived products should be handled at BSL-2 levels (biosafety level 2) or higher as recommended in the CDC-NIH manual, "微生物和生物医学实验室的生物安全,” 1998. For further assistance, please contact your site Safety Officer or yobo体育 technical service.
批不合格通知书: 如果组织批次没有通过我们的质量控制或无菌测试, 我们会通知顾客，并免费更换纸巾. 因为我们的QC和无菌检测是在出货后进行的, 通知会尽快发出(一般情况下, ET-50 failures will be notified by Wednesday 5pm; sterility failures will be notified within 8 days of shipment).
